Human hepatocytes in mice receiving pre-immune injection with human cord blood cells |
| |
Authors: | Paolo Turrini Josè Gonzalez Giuseppina Bonanno Nadia Rosenthal Ralph Laufer |
| |
Institution: | a Pharmacology Department, Istituto di Ricerca di Biologia Molecolare “P.Angeletti” (IRBM), Merck Sharp and Dohme Research Laboratories, Rome, Italy b Department of Anatomy, Catholic University of Sacred Heart, Rome, Italy c Department of Obstetrics and Gynecology, Catholic University of Sacred Heart, Rome, Italy d EMBL Monterotondo, Mouse Biology Programme, via Ramarini 32, 00016 Monterotondo—Scalo (RM), Italy |
| |
Abstract: | It is well established that certain subpopulations of human adult stem cells can generate hepatocyte-like cells when transplanted into adult immunosuppressed mice. In the present study, we wanted to explore whether xeno-transplantation of human cord blood CD34+ (hCBCD34+) cells during pre-immune stages of development in immunocompetent mice might also lead to human-mouse liver chimerism. Freshly isolated hCBCD34+ cells were xeno-transplanted into non-immunosuppressed mice by both intra-blastocyst and intra-fetal injections. One and four weeks after birth, immunostaining for different human-specific hepatocyte markers: human hepatocyte-specific antigen, human serum albumin, and human α-1-antitrypsin indicated the presence of human hepatocyte-like cells in the livers of transplanted animals. Detection of human albumin mRNA further corroborated the development of pre-immune human-mouse chimeras. The current report, besides providing new evidence of the potential of hCBCD34+ cells to generate human hepatocyte-like cells, suggests novel strategies for generating immunocompetent mice harboring humanized liver. |
| |
Keywords: | Xeno-transplantation Stem cells Intra-fetal Intra-blastocyst Liver Hepatocytes |
本文献已被 ScienceDirect 等数据库收录! |
|