Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNA(Met) |
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| Authors: | J Bratty T F Wu K Nicoghosian K K Ogilvie J P Perreault G Keith R Cedergren |
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| Affiliation: | Département de Biochimie, Université de Montréal, Qué, Canada. |
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| Abstract: | A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNA(Met) sample and threonylated to 76% of the level observed with native tRNA(fMet) from E. coli. |
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