Polyamines appear to be second messengers in mediating Ca2+ fluxes and neurotransmitter release in potassium-depolarized synaptosomes |
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| Authors: | Z Iqbal H Koenig |
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| Affiliation: | 1. Neurology Service, VA Lakeside Medical Center, 333 East Huron Street, Chicago, IL 60611 USA;2. Department of Neurology, Northwestern University Medical School, Chicago, IL 60611 USA;3. Department of Biochemistry, Northwestern University Medical School, Chicago, IL 60611 USA;1. Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany;2. Structural Bioinformatics, BIOTEC TU Dresden, Tatzberg 47-51, Dresden 01307, Germany;3. Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstraße 16-18, D-04107 Leipzig, Germany;4. Department of Metabolomics, Helmholtz-Centre for Environmental Research – UFZ, Permoserstraße 15, 04318 Leipzig, Germany;5. Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg East, Denmark;1. Department of Chemistry, Wayne State University, Detroit, MI 48202, United States;2. Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States;1. Department of Biochemistry and Molecular Biology, Building Severo Ochoa, 2nd Floor, Campus de Rabanales, University of Córdoba, 14014 Córdoba, Spain;2. Department of Agricultural Chemistry, Building Marie Curie, 3rd Floor, Campus de Rabanales, University of Córdoba, 14014 Córdoba, Spain;3. Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, University of Córdoba, 14014 Córdoba, Spain |
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| Abstract: | High potassium (50 mM) depolarization induces a rapid (less than 15 sec) increase in the levels of the polyamines putrescine, spermidine and spermine and their rate-regulating synthetic enzyme ornithine decarboxylase in synaptosomes from rat cerebral cortex. The ornithine decarboxylase inhibitor alpha-difluoromethylornithine blocked the K+-stimulated increase in enzyme activity and polyamines and also suppressed the increase in 45Ca2+ influx and efflux and the Ca2+-dependent release of GABA and norepinephrine. Added putrescine attenuated or negated the effects of alpha-difluoromethylornithine. These results suggest that enhanced polyamine synthesis is required for potassium depolarized stimulation of synaptic function. |
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