A simple mechanism decreasing free metabolite pool size in static spatial channelling |
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| Authors: | Korzeniewski Bernard Quant Patti A. |
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| Affiliation: | (1) Department of Biochemistry, University of Cambridge, Cambridge, England;(2) Institute of Molecular Biology, Jagiellonian University, Kraków, Poland;(3) Paedriatric Surgical Unit, Division of Clinical Sciences, Institute of Child Health and Great Ormond Street Hospital for Children NHS Trust, University of London, England |
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| Abstract: | We propose a simple mechanism which enables decrease of the free pool of channelled metabolite in static spatial channelling, when the concentration of the enzyme consuming the channelled metabolite is greater than the concentration of the enzyme producing this metabolite. Spatial channelling occurs between two enzymes when the common metabolite is released to a small space between these enzymes and does not form a ternary covalent complex with them, as is the case in covalent (dynamic or static) channelling. The mechanism proposed is qualitatively independent of rate constants, metabolite concentrations as well as other kinetic properties and is quantitatively significant for all physiologically relevant conditions. Calculations show that the free metabolite pool must decrease, when the concentration of the enzyme consuming the channelled metabolite is greater than the enzyme producing it. This mechanism is much more effective than increase in the concentration (or rate constant) of the enzyme consuming the metabolite in the absence of spatial channelling. |
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| Keywords: | channelling metabolite concentration enzyme-enzyme interaction kinetic model |
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