Characterization of an inducible mannitol-binding protein fromPseudomonas aeruginosa |
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Authors: | Robert C. Eisenberg Paul V. Phibbs Jr. |
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Affiliation: | (1) Department of Microbiology, Medical College of Virginia, Virginia Commonwealth University, 23298 Richmond, Virginia, USA |
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Abstract: | Pseudomonas aeruginosa strain PAO formed a mannitol-binding protein that was coinducible with mannitol uptake activity. One species of mannitol-binding protein was isolated from disrupted cell suspensions and the protein also was released from mannitol-grown cells by osmotic shock treatment. The mannitol-binding protein )molecular weight 37,000) was purified 49-fold and resolved from the enzyme mannitol dehydrogenase (molecular weight 85,000), which did not exhibit mannitol-binding activity. Mannitol-binding protein had an isoelectric point (pI) of 8.3 and an apparent dissociation constant (KD) of 2.3 M for mannitol. Mannitol binding was unaffected by glucose and glycerol in competitive inhibition studies, although mannose, fructose, and sorbitol caused 5 to 20% inhibition of binding at concentrations equimolar with mannitol. |
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