The formation of iron-binding fragments of hen ovotransferrin by limited proteolysis |
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| Authors: | John Williams |
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| Affiliation: | Molecular Enzymology Laboratory, Department of Biochemistry, University of Bristol, Bristol BS8 1TD., U.K. |
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| Abstract: | 1. Iron was added to hen ovotransferrin to 30% saturation and the protein was digested with trypsin or chymotrypsin. 2. Iron-binding fragments were isolated. They carried one atom of iron/mol (mol.wt. 35000) and consisted of a single polypeptide chain derived from the N-terminal half of the protein. Carbohydrate was not present. 3. The fragments were able to bind a variety of metals and to donate iron to reticulocytes. |
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