1H, 13C and 15N backbone and side chain resonance assignments of a Myxococcus xanthus anti-repressor with no known sequence homologues |
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Authors: | Esther León Carlos González Montserrat Elías-Arnanz S. Padmanabhan M. Angeles Jiménez |
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Affiliation: | (1) Instituto de Química Física Rocasolano, CSIC, 28006 Madrid, Spain;(2) Departamento de Genética y Microbiología, área de Genética (Unidad Asociada al IQFR-CSIC), Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain |
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Abstract: | The CarS antirepressor activates a photo-inducible promoter in Myxococcus xanthus by physically interacting with the CarA repressor and eliminating the latter’s binding to operator DNA. Interestingly, interactions with both CarS and operator are crucially dependent on the DNA recognition helix of the CarA winged-helix DNA-binding domain. The CarA–CarS and the CarA-operator interfaces therefore overlap, and CarS may have structural features that mimic operator DNA. CarS has no known sequence homologues and its Gly and Pro contents are unusually high. Here, we report 1H, 13C and 15N backbone and side chain assignments of CarS1, an 86-residue truncated yet fully functional variant of CarS. Secondary structural elements inferred from these data differ from those predicted from sequence. |
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Keywords: | Antirepressor CarA Carotenogenesis CarS Myxococcus xanthus NMR |
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