Profiles of fatty acids and triacylglycerols and their influence on the anaerobic biodegradability of effluents from poultry slaughterhouse

The hydrolysis of effluent from a poultry slaughterhouse containing 800 mg oil and grease (O&G)/L was conducted with 1% (w/v) of an enzymatic pool obtained by solid-state fermentation with the fungus Penicillium restrictum. The chromatographic evaluation of the lipid profile during hydrolysis indicated a higher concentration of acids after 4 h of reaction (2954 mg/L), with a predominance of oleic, palmitic, and linoleic acids. Effluent aliquots were collected after 4, 8, and 24 h of hydrolysis and tested for anaerobic biodegradation in sequential batches. An adaptation of the biomass was observed, both in the control experiment (with non-hydrolyzed raw effluent) and in the experiments with enzymatically pre-treated effluent. The specific methane production in the control experiment was 0.248 L CH₄/g COD_consumed, and in the experiment with effluent pre-treated for 4 h, this production was 0.393 L CH₄/g COD_consumed, indicating a higher methane production after enzymatic hydrolysis.