Circular dichroism properties of ethidium bromide-deoxyribonucleic acid complexes |
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| Authors: | S Aktipis W W Martz |
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| Affiliation: | 1. Faculty of Chemistry, Adam Mickiewicz University, 61-614 Poznan, Uniwersytetu Poznanskiego 8, Poland;2. Institute of Molecular Physics, Polish Academy of Sciences, 60-179 Poznan, Smoluchowskiego 17, Poland;1. Department of Chemistry and Chemical Engineering, Physical Chemistry, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden;2. CIQSO - Center for Research in Sustainable Chemistry and Department of Chemistry, University of Huelva, Campus de El Carmen s/n, E-21071 Huelva, Spain;1. Department of Chemistry, Sri Eshwar College of Engineering, Coimbatore 641 202, India;2. Department of Chemistry, Sri GVG Visalakshi College for Women, Udumalpet 642128, India;3. Department of Chemistry, Bannari Amman Institute of Technology, Sathyamangalam 638 401, India;4. Nanotechnology Research & Education Centre, South Ural State University, Chelyabinsk 454 080, Russia;5. Department of Chemistry, Fu Jen Catholic University, New Taipei City 242, Taiwan |
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| Abstract: | Ethidium bromide (EB) exhibits upon interaction with DNA induced optical activity between 290 to 400mμ and 440 to 540mμ. The dependence of circular dichroism on temperature and EB to DNA molar ratios indicates that “nearest-neighbor” interactions between ethidium molecules positioned within segments of the helix are the main source of optical activity in the 290 to 350mμ region. Optical activity between 440 to 540mμ may be induced by isolated ethidium molecules, interacting with double stranded DNA. |
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