Interspecific genetic linkage map, segregation distortion and genetic conversion in coffee (Coffea sp.) |
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| Authors: | C L Ky P Barre M Lorieux P Trouslot S Akaffou J Louarn A Charrier S Hamon M Noirot |
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| Institution: | (1) GeneTrop, Centre IRD de Montpellier, B.P. 5045, 34032 Montpellier cedex 1, France Fax: +33467416222, FR;(2) Station ORSTOM de Génétique du caféier, Man, B.P. 434, Ivory Coast,;(3) ENSA, UFR Génétique et Amélioration des Plantes, Place Viala, F-34060 Montpellier, France, FR |
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| Abstract: | An interspecific partial genetic linkage map of Coffea sp. based on 62 backcross hybrids is presented. F1 hybrids were generated by a cross between the wild C. pseudozanguebariae and the anciently cultivated C. liberica var. dewevrei (DEW); progeny were then derived from a backcross between F1 hybrid and DEW. The map construction consisted of a two-step strategy using 5.5 and 3.1 LOD scores revealed by simulation
file. The map consisted of 181 loci: 167 amplified fragment length polymorphism (AFLP) and 13 random fragment length polymorphism
(RFLP) loci. The markers were assembled into 14 linkage groups, each with 4–31 markers covering 1,144 cM. Segregation distortion
was observed for 30% of all loci, in particular 3:1 and 1:3 ratios equally favouring each of the two parents. The existence
of such ratios suggests genetic conversion events. This map also represents an initial step towards the detection of quantitative
trait loci.
Received: 4 Janaury 2000 / Accepted: 17 January 2000 |
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| Keywords: | Coffea sp AFLPs RFLPs Linkage map Segregation distortion Genetic conversion |
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