Differentiating effect of L-tyrosine on the human melanoma cell line IIB-MEL-J |
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Authors: | L Guerra L Bover J Mordoh |
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Affiliation: | Instituto de Investigaciones Bioquimicas Luis F. Leloir Fundacion Campomar, Buenos Aires, Argentina. |
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Abstract: | IIB-MEL-J is a highly heterogeneous newly established human melanoma cell line. The addition of 3 mM L-tyrosine to the culture medium produced (1) a great decrease in the cell growth rate, (2) a loss of the anchor-age-independent growth capacity, and (3) a change in the morphology of the cells to a fibroblastoid aspect. Coincident with these changes, an increase in subpopulations I and II and a decrease in subpopulations III and IV took place. In view of this evidence we consider that the cells have differentiated. The melanin production was not increased by the L-tyrosine treatment, suggesting that differentiation and melanin expression are not strictly correlated. |
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