A catalog of human cDNA expression clones and its application to structural genomics |
| |
| Authors: | Konrad Büssow Claudia Quedenau Volker Sievert Janett Tischer Christoph Scheich Harald Seitz Brigitte Hieke Frank H Niesen Frank Götz Ulrich Harttig Hans Lehrach |
| |
| Affiliation: | Konrad Büssow, Claudia Quedenau, Volker Sievert, Janett Tischer, Christoph Scheich, Harald Seitz, Brigitte Hieke, Frank H Niesen, Frank Götz, Ulrich Harttig, and Hans Lehrach |
| |
| Abstract: | We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures. |
| |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
