A novel membrane based process to isolate photosystem-I membrane complex from spinach |
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| Authors: | Jianguo Liu Mengmeng Yin Meng Wang Xuefang Zhang Baosheng Ge Shuang Liu Jianren Lu Zhanfeng Cui |
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| Affiliation: | (1) Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266555, People’s Republic of China;(2) School of Physics, Manchester University, Manchester, M60 1QD, UK;(3) Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK |
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| Abstract: | The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300 kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 and n-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I were also studied using an O2 electrode. Under optimized conditions, the PS-I purity obtained in the retentate was about 84% and the activity recovery was greater than 94% after ultrafiltration. To our knowledge, this is the first report of the isolation of a membrane protein using ultrafiltration alone. |
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