发酵条件对纤维素分解菌酶活性的影响研究*

研究了4株可用作饲料添加剂的纤维素分解菌，在不同发酵时间、发酵温度和厌氧发酵条件下，菌体蛋白含量、纤维素酶和半纤维素酶活性等的变化。结果表明发酵20h酶活性最高；供试菌都适宜在28℃恒温发酵；在厌氧条件下，39℃士2℃发酵12h、24h、36h，供试菌均可在PDA平板上正常生长，发酵48h供试菌生长和酶活均受不同程度的影响。研究为菌株的生产、保存和应用提供了重要依据。

THE EFFECT OF FERMENTATION ON CELLULOLYTIC ENZYME ACTIVITY

Four cellulolytic strains, which can be used as feed additive, were studied under the conditions of various temperature, incubation time, and anaerobic process, and examined the changes of their cell protein content, cellulase and hemi-cellulase activity. The results show: 1) The maximum cellulolytic enzyme activities were observed incubation 20h; 2) Constant medium temperature 28℃ was adequate to the growth of the 4 strains ; 3) anaerobic condition, 39℃±2℃ and fermentation 12h, 24h, 36h, the tested strains can growth well in PDA plate, however, the cellulolytic enzyme activities and growth of the tested strains were influenced adversely when fermentation 48h. The experiment provide many important basis for the strains production, storage and utilization.