Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets |
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| Authors: | Yann Froelicher Jean-Baptiste Bassene Emna Jedidi-Neji Dominique Dambier Raphael Morillon Geneviève Bernardini Gilles Costantino Patrick Ollitrault |
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| Institution: | (1) Unité de Recherche Multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), San Giuliano, 20230, France;(2) Laboratoire de physiologie et biotechnologie végétale, Institut National de Recherche Agronomique de Tunisie, Ariana, 2049, Tunisia;(3) Unité de Recherche Multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier, 34398, France;(4) Unité de Génétique et écophysiologie de la qualité des agrumes, Institut National de Recherche Agronomique (INRA), San Giuliano, 20230, France |
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| Abstract: | This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of ‘Meyer’
lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300,
600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level
of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid
parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants
were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of ‘Fortune’ (Citrus clementina Hort ex Tan. × Citrus tangerina Hort ex Tan.) and ‘Ellendale’ (Citrus reticulata Blanco × Citrus sinensis L. Osb) is reported here for the first time. |
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| Keywords: | Citrus Rutaceae Induced gynogenesis Haploid STMS markers |
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