Molecular identification of house dust mites and storage mites |
| |
Authors: | Shew Fung Wong Ai Ling Chong Joon Wah Mak Jessie Tan Suk Jiun Ling Tze Ming Ho |
| |
Institution: | (1) International Medical University, No. 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Kuala Lumpur, Malaysia;(2) Acarology Unit, Institute for Medical Research, 50588 Kuala Lumpur, Malaysia |
| |
Abstract: | Mites are known causes of allergic diseases. Currently, identification of mites based on morphology is difficult if only one
mite is isolated from a (dust) sample, or when only one gender is found, or when the specimen is not intact especially with
the loss of the legs. The purpose of this study was to use polymerase chain reaction–restriction fragment length polymorphism
(PCR–RFLP) of the ITS2 gene, to complement the morphological data for the identification of mites to the species level. For
this, six species were cultured: Dermatophagoides pteronyssinus, D. farinae, Blomia tropicalis, Tyrophagus putrescentiae, Aleuroglyphus ovatus and Glycycometus malaysiensis. Genomic DNA of the mites was extracted, quantified, amplified and digested individually with restriction enzymes. Hinf I
and Ple I differentiated the restriction patterns of D. pteronyssinus and D. farinae. Bfa I and Alu I enzymes differentiated B. tropicalis and G. malaysiensis. Ple I enzyme was useful for the differentiation between T. putrescentiae and A. ovatus. Bfa I was useful for the differentiation of G. malaysiensis from the rest of the species. In conclusion, different species of mites can be differentiated using PCR–RFLP of ITS2 region.
With the established PCR–RFLP method in this study, identification of these mites to the species level is possible even if
complete and intact adult specimens of both sexes are not available. As no study to date has reported PCR–RFLP method for
the identification of domestic mites, the established method should be validated for the identification of other species of
mites that were not included in this study. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|