Involvement of Matrix Metalloproteinases on the Inhibition of Cells Invasion and Migration by Emodin in Human Neuroblastoma SH-SY5Y Cells |
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| Authors: | Hsu-Feng Lu Kuang-Chi Lai Shu-Chun Hsu Hui-Ju Lin Chao-Lin Kuo Ching-Lung Liao Jai-Sing Yang Jing-Gung Chung |
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| Institution: | (1) Department of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipei, Taiwan, ROC;(2) College of Human Ecology, Fu-Jen University, Taipei, Taiwan, ROC;(3) School of Medicine, China Medical University, Taichung, Taiwan, ROC;(4) Department of Surgery, China Medical University Beigang Hospital, Yunlin, Taiwan, ROC;(5) Department of Chinese Medicine Resources, China Medical University, Taipei, Taiwan, ROC;(6) Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan, ROC;(7) Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan, ROC;(8) Department of Pharmacology, China Medical University, Taichung, Taiwan, ROC;(9) Department of Biological Science and Technology, China Medical University, No 91, Hsueh-Shih Road, Taichung, 404, Taiwan, ROC;(10) Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan, ROC; |
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| Abstract: | Emodin (1,3,8-trihydroxy-6-methylanthaquinone), an active component present in the root and rhizome of Rheum palmatum L. (Polygonaceae) has anti-bacterial, anti-tumor, diuretic and vasorelaxant effects. However, its mechanism of action on
the cell migration and invasion of human neuroblastoma cancer SH-SY5Y cells is not fully understood. In this study, firstly,
the effects of emodin on the percentage of viable cells were examined by using MTT assay and it was found that emodin induced
dose-and time-dependent inhibition in human neuroblastoma SH-SY5Y cells. Second, the effects of emodin on the migration and
invasion of SH-SY5Y cells were examined by using wound assay and matrigel counting and the results showed that emodin suppressed
the migration and invasion of SH-SY5Y cells. Third, we examined the effect of emodin on the levels of associated proteins
by using Western blotting and the results indicated that emodin inhibited the levels of GRB2, RhoA, HIF-1α, VEGF, FAK, iNOS,
COX2, p-p38, p-c-jun, MMP2, MMP9 and MMP7 but promoted the levels of PKC, PI3K, MEKK3 and NF-κB p65 that led to the inhibition
of migration and invasion of SH-SY5Y cells in vitro. |
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| Keywords: | Emodin Invasion Migration MMPs SH-SY5Y cells |
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