Molecular characterization of Cryptococcus gattii genotype AFLP6/VGII isolated from woody debris of divi-divi (Caesalpinia coriaria), Bonaire,Dutch Caribbean |
| |
Institution: | 1. Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands;2. Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands;3. Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India;4. Department of Pediatric Pulmonology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands;1. Department of Nuclear Medicine, Odense University Hospital, Søndre Boulevard 29, 5000 Odense, Denmark;2. Department of Radiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands;1. Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, United States;2. Department of Physical Therapy, Marquette University, Milwaukee, WI 53226, United States;3. Neuroscience Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, United States;4. Zablocki Veterans Affairs Medical Center, Milwaukee, WI 53226, United States |
| |
Abstract: | BackgroundThe basidiomycetous yeast Cryptococcus gattii is an emerging and primary pathogen. There is a lack of information about its environmental spread outside outbreak regions in Mediterranean Europe, North and South America. Environmental sampling for C. gattii and molecular characterization of the obtained isolates will provide an insight into the global spread of the various genotypes.MethodsWoody debris of native divi-divi (Caesalpinia coriaria) trees were sampled across Bonaire, Dutch Caribbean. Colonies suspected for Cryptococcus species were subjected to standard mycology investigations and identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Isolates identified as C. gattii were subjected to amplified fragment length polymorphism genotyping, mating-type analysis and multi-locus sequence typing.ResultsTen colonies of C. gattii were cultured from different trunk hollows of the same divi-divi tree. Molecular characterization showed that all isolates were genotype AFLP6/VGII and mating-type α. Multi-locus sequence typing revealed that all isolates were genetically indistinguishable from each other.ConclusionsC. gattii is present in the environment of Bonaire, which suggests that this yeast is likely to be present in the environment of other Caribbean islands. |
| |
Keywords: | Multi-locus sequence typing Amplified fragment length polymorphism fingerprinting Environmental sampling Tipado por secuenciación de múltiples loci Polimorfismo de tamaño de fragmentos amplificados Muestreo medioambiental |
本文献已被 ScienceDirect 等数据库收录! |
|