Biochemical mechanism of chlorantraniliprole resistance in Spodoptera litura (Fab) (Lepidoptera: Noctuidae) |
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| Institution: | 1. USDA-ARS-JWDSRC, Stoneville, MS 38776, USA;2. USDA, Animal and Plant Health Inspection Service, Biotechnology Regulatory Services, Riverdale, MD 20737, USA;3. USDA-ARS, Poplarville, MS, USA;4. Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA;1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, China;2. Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning Province 125100, China;3. Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | Spodoptera litura, a polyphagus insect pest of economic importance, having the ability to develop resistance to various classes of insecticides was selected for the study. Leaf dip bioassay studies were done after ten generations of selection pressure reported the development of resistance up to 80 folds by S. litura against chlorantraniliprole compared to lab susceptible strain. Bioassay studies conducted using enzyme inhibitors such as triphenyl phosphate (TPP) followed by diethyl-maleate (DEM) and piperonyl butoxide (PBO) showed good amount of synergism with chlorantraniliprole and improved efficacy against resistant strain. Findings of bioassay studies were supported by in-vitro enzyme inhibition assays. Esterase activity in gut homogenate of resistant strain was significantly inhibited by TPP suggesting esterase mediated biochemical resistance development in S. litura against chlorantraniliprole. |
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