Evaluation of parasitological and molecular techniques for the diagnosis
and assessment of cure of schistosomiasis mansoni in a low transmission
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Authors: | Liliane Maria Vidal Siqueira Luciana Inácia Gomes Edward Oliveira Eduardo Ribeiro de Oliveira áureo Almeida de Oliveira Martin Johannes Enk Nídia Figueiredo Carneiro Ana Rabello Paulo Marcos Zech Coelho |
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Institution: | 1.Laboratório de Esquistossomose;2.Laboratório de Pesquisas Clínicas, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, Brasil;3.Instituto Evandro Chagas, Ananindeua, PA, Brasil;4.Centro de Controle de Zoonoses, Montes Claros, MG, Brasil |
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Abstract: | This study evaluated parasitological and molecular techniques for the diagnosis and
assessment of cure of schistosomiasis mansoni. A population-based study was performed
in 201 inhabitants from a low transmission locality named Pedra Preta, municipality
of Montes Claros, state of Minas Gerais, Brazil. Four stool samples were analysed
using two techniques, the Kato-Katz® (KK) technique (18 slides) and the
TF-Test®, to establish the infection rate. The positivity rate of 18 KK
slides of four stool samples was 28.9% (58/201) and the combined parasitological
techniques (KK+TF-Test®) produced a 35.8% positivity rate (72/201).
Furthermore, a polymerase chain reaction (PCR)-ELISA assay produced a positivity rate
of 23.4% (47/201) using the first sample. All 72 patients with positive
parasitological exams were treated with a single dose of Praziquantel® and
these patients were followed-up 30, 90 and 180 days after treatment to establish the
cure rate. Cure rates obtained by the analysis of 12 KK slides were 100%, 100% and
98.4% at 30, 90 and 180 days after treatment, respectively. PCR-ELISA revealed cure
rates of 98.5%, 95.5% and 96.5%, respectively. The diagnostic and assessment of cure
for schistosomiasis may require an increased number of KK slides or a test with
higher sensitivity, such as PCR-ELISA, in situations of very low parasite load, such
as after therapeutic interventions. |
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Keywords: | Schistosoma mansoni schistosomiasis diagnosis control low transmission area Brazil |
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