Molecular diagnosis of endemic and invasive mycoses: Advances and challenges |
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| Affiliation: | 1. School of Medicine and Health Sciences, Universidad del Rosario, Bogota, Colombia;2. Medical and Experimental Mycology Group, Corporación para Investigaciones Biológicas (CIB), Medellin, Colombia;1. Hematology, Graduate School of Medicine, Osaka City University, Osaka, Japan;2. Research and Development Center, Fuso Pharmaceutical Industries, Ltd., Osaka, Japan;1. NeuroCenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland;2. Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland;3. Institute of Parasitology, Vetsuisse Faculty and Medical Faculty, University of Bern, Bern, Switzerland;4. Canadian Food Inspection Agency, Lethbridge Laboratory, Lethbridge, Alberta, Canada;1. Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia;2. Medical Faculty, Slovak Medical University, Limbová 12, 833 03 Bratislava, Slovakia;1. Grupo de Investigación de Salud Global, Universidad de Cantabria, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander, Cantabria, España;2. Servicio de Neumología, Hospital de Laredo, Cantabria, España;3. Servicio de Neumología-Debilidad muscular y caquexia en las enfermedades respiratorias crónicas y el cáncer de pulmón, IMIM-Hospital del Mar, Barcelona, España;4. Departament de Ciències Experimentals i de la Salut (CEXS), Universitat Pompeu Fabra (UPF), Parc de Recerca Biomèdica de Barcelona (PRBB), Barcelona, España;5. Centro de Investigación en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII), Barcelona, España |
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| Abstract: | The diagnosis of endemic and invasive fungal disease remains challenging. Molecular techniques for identification of fungi now play a significant and growing role in clinical mycology and offer distinct advantages as they are faster, more sensitive and more specific. The aim of this mini-review is to provide an overview of the state of the art of molecular diagnosis of endemic and invasive fungal diseases, and to emphasize the challenges and current need for standardization of the different methods. The European Aspergillus PCR Initiative (EAPCRI) has made significant progress in developing a standard for Aspergillus polymerase chain reaction (PCR), but recognizes that the process will not be finished until clinical utility has been established in formal and extensive clinical trials. Similar efforts should be implemented for the diagnosis of the other mycoses in order to fully validate the current methods or reinforce the need to design new ones.This manuscript is part of the series of works presented at the “V International Workshop: Molecular genetic approaches to the study of human pathogenic fungi” (Oaxaca, Mexico, 2012). |
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| Keywords: | Endemic mycosis Invasive mycoses Polymerase chain reaction Molecular diagnosis Standardization Micosis endémicas Micosis invasivas Reacción en cadena de la polimerasa Diagnóstico molecular Estandarización |
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