The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture |
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Authors: | Jeremy R. Mason |
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Affiliation: | (1) Division of Life Sciences, King's College London, Campden Hill Road, W8 7AH London, UK |
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Abstract: | The oxidation of catechol, an intermediate in benzene catabolism, was studied using transient variations in dissolved oxygen tension (DOT) when a succinate limited steady state culture of Pseudomonas putida ML2 was perturbed with a pulse of another substrate. A model was developed and tested for the effect of fluctuations in oxidizing enzyme activity on DOT. It was found that the rate of induction of catechol oxidizing enzymes was independent of dilution rate up to a relative growth rate /max of 0.75. Only at higher dilution rates was catabolite repression observed.Abbreviations DOT dissolved oxygen tension - KLa gas transfer coefficient - specific growth rate - max maximum specific growth rate - Ks substrate saturation constant |
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Keywords: | Pseudomonas Benzene catabolism Catechol dioxygenase continuous culture Catabolite repression |
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