栽培与野生化血丹不同部位中两种化学成分含量分析

为探讨并分析栽培与野生化血丹植株中不同部位中两种化学成分的含量差异,该研究采用超声法提取、高效液相色谱法(HPLC)测定栽培与野生化血丹根、茎、叶、花、混合样等部位中桃叶珊瑚苷和梓醇的含量,并进行比较。结果表明:(1)桃叶珊瑚苷在栽培与野生化血丹植株内均有分布,含量均以根中最高,其在栽培与野生化血丹植株内的含量表现分别为根叶混合样茎花、根混合样茎花叶,栽培化血丹不同部位中桃叶珊瑚苷的含量均高于野生化血丹。(2)梓醇在栽培化血丹的茎中未检出,在栽培与野生化血丹其他部位均有分布,含量均以叶中最高,其在栽培与野生化血丹植株内的含量分别表现为叶花混合样根、叶混合样茎花根,野生化血丹不同部位中梓醇的含量均高于栽培化血丹。(3)桃叶珊瑚苷和梓醇在栽培和野生化血丹植株不同部位中的含量均存在显著差异(P0.05),栽培与野生同一部位间总体上无显著差异,为该濒危药用植物资源药用部位选择和合理开发利用提供实验参考。

Content analysis of two chemical compositions in different parts of cultivated and wild Centranthera grandiflora

To study and analysis the difference contents of two active ingredients in different parts of cultivated and wild Centranthera grandiflora, the contents of aucubin and catalpol in roots, stems, leaves, flowers and mixed samples of cultivated and wild C. grandiflora were determined by ultrasonic extraction and high performance liquid chromatography(HPLC)and compared. The results were as follows:(1)Aucubin was distributed in both cultivated and wild plants, with the highest contents in roots. The contents in cultivated and wild C. grandiflora were root > leaf > mixed sample > stem > flower, root > mixed sample > stem > flower > leaf, respectively. The content of aucubin in different parts of cultivated plants was higher than that in wild plants.(2)Catalpol was not detected in the stem of cultivated C. grandiflora, but was distributed in other parts of cultivated and wild C. grandiflora, with the highest content in leaves. The contents of catalpol in cultivated and wild C. grandiflora were leaf > flower > mixed sample > root, leaf > mixed sample > stem > flower > roots, respectively. The contents of catalpol in different parts of wild were higher than those of cultivated C. grandiflora.(3)There were significant differences(P<0.05)in the contents of aucubin and catalpol in different parts of cultivated and wild C. grandiflora plants, and there was no significant differences between cultivated and wild parts on the whole, which provides experimental reference for the selection and reasonable utilization of the medicinal site of the endangered medicinal plant resources