Chemical and immunochemical characterisation of substance P-like immunoreactivity and physalaemin-like immunoreactivity in a carcinoid tumour |
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| Authors: | J.M. Conlon G. Schäfer W.E. Schmidt L.H. Lazarus H.D. Becker W. Creutzfeldt |
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| Affiliation: | 1. Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-Gesellschaft at the Department of Medicine, Goßlerstraße 10d, D-3400 Göttingen, F.R.G.;2. Division of Gastroenterology and Metabolism at the Department of Medicine, University of Göttingen, Robert-Koch-Straße 40, D-3400 Göttingen, F.R.G.;3. Laboratory of Behavioral and Neurological Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, U.S.A.;4. Department of Surgery, University of Göttingen, Robert-Koch-Straße 40, D-3400 Göttingen, F.R.G. |
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| Abstract: | Extracts of a carcinoid tumour, resected from the mid-portion of the ileum of a patient with no symptoms of endocrine disorder, were associated with a high concentration of substance P-like immunoreactivity. Using reverse-phase high performance liquid chromatography and antisera specifically directed against the C-terminal and N-terminal regions of substance P and against the N-terminal region of physalaemin, the following components were isolated and identified: substance P and its oxidised form, [pGlu5]substance P-(5–11) peptide and its oxidised form, and the oxidised form of physalaemin. The identity of tumour substance P with the undecapeptide was confirmed by amino acid analysis and high performance ion-exchange chromatography. In vitro incubation of tumour tissue from a lymph node metastasis from the same patient with [3H]leucine resulted in incorporation of radioactivity into newly synthesised substance P. |
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| Keywords: | HPLC radioimmunoassay biosynthesis tachykinin neurokinin |
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