Isolation of the facA (acetyl-CoA synthetase) gene of Phycomyces blakesleeanus |
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Authors: | Victoriano Garre Francisco J. Murillo Santiago Torres-Martínez |
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Affiliation: | (1) Departamento de Genética y Microbiología, Facultad de Biologia, Universidad de Murcia, Apdo 4021, 30071 Murcia, Spain |
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Abstract: | A 5.6 kb DNA fragment from the fungus Phycomyces blakesleeanus has been cloned and sequenced. The fragment contains a gene that probably codes for the enzyme acetyl-coenzyme A synthetase (facA). The amino acid sequence deduced for the P. blakesleeanns protein is highly homologous to those of acetyl-coA-synthetases from other organisms. When placed under the control of a constitutive promoter from Aspergillus nidulans, the cloned gene complemented a facA mutation of this organism. In P. blakesleeanns, the expression of facA is induced by acetate. |
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Keywords: | Filamentous fungus Acetate Transgenic expression |
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