Effect of exogenous gibberellic acid,abscisic acid,and benzylaminopurine on epicotyl dormancy of cultured herbaceous peony embryos |
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| Authors: | Julie A T Buchheim Len F Burkhart Martin M Meyer Jr |
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| Institution: | (1) Department of Horticulture, University of Illinois, 61801 Urbana, IL, USA;(2) Present address: Department of Biological Science, University of Tulsa, 74104 Tulsa, OK, USA;(3) Present address: Davey Tree Expert Co., 44240 Kent, OH, USA |
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| Abstract: | Epicotyl dormancy was broken in cultured peony (Paeonia lactiflora Pall.) embryos after topical application of agarose gels containing gibberellic acid, with optimum growth at 1.5 mM gibberellic acid. Addition of 100  M abscisic acid to the medium resulted in complete inhibition of gibberellic acid-stimulated promotion of dormant epicotyls. Epicotyl dormancy was also broken in embryos by culture on media containing 1 or 10 M benzylaminopurine. A highly significant increase in leaf number occurred when embryos were both cultured on medium containing benzylaminopurine and treated topically with gibberellic acid. Anatomical and morphological studies indicated that the increase in shoot growth was due to the development and growth of 1) buds formed at the cotyledonary node, 2) axillary buds, and 3) adventitious meristems originating from subepidermal parenchymatous tissue.Abbreviations ABA
abscisic acid
- BA
N6-benzylaminopurine
- DMSO
dimethyl sulfoxide
- GA3
gibberellic acid
- LS
Linsmaier and Skoog |
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| Keywords: | embryo culture image analysis plant growth regulators seed germination tissue culture |
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