Regeneration of fertile plants from embryogenic suspension culture protoplasts of Sorghum vulgare

Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4–5 days of culture, and achieved 16.8% division frequency by 10 days. Protocalli proliferated further upon transfer to C₁ solid medium. After that, they were moved to C₁ differentiation medium to induce shoot formation, followed by whole plant regeneration. So far, 60 plants have been obtained, with only two albinos. Some of these have been transplanted to soil in pots and grown to flowering and have set seeds.Abbreviations BA 6-benzylaminopurine - CH casein hydrolysate - 2,4-D 2,4-dichlorophenoxyacetic acid - IAA 3-indoleacetic acid - IBA 3-indolebutyric acid - KT kinetin - PVP polyvinylpyrrolidone