The lamellar to hexagonal phase transition in phosphatidylethanolamine liposomes: a fluorescence anisotropy study |
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| Authors: | J L Nieva J Castresana A Alonso |
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| Affiliation: | Department of Biochemistry, Faculty of Science, University of the Basque Country, Bilbao, Spain. |
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| Abstract: | The steady-state anisotropy of trimethylammonium diphenylhexatriene fluorescence has been used to monitor the thermotropic lamellar to HII hexagonal phase transition in an unsaturated phosphatidylethanolamine. The transition is observed in lipid aggregates when they are heated above the transition temperature Th, as well as in diluted liposomes after aggregation above Th. Changes in fluorescence anisotropy are not observed with Ca(++)-induced fusion of phosphatidylserine vesicles, a process not involving hexagonal phase formation. |
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