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DNA recognition and cleavage by the EcoP15 restriction endonuclease.
Authors:Sheikh M Hadi  Brigitte Bächi  John CW Shepherd  Robert Yuan  Kurt Ineichen  Thomas A Bickle
Institution:Biozentrum der Universität Basel Klingelbergstrasse 70 CH-4056 Basel, Switzerland
Abstract:EcoP15 is a restriction-modification enzyme coded by the P15 plasmid of Escherichia coli. We have determined the sites recognized by this enzyme on pBR322 and simian virus 40 DNA. The enzyme recognizes the sequence:
/></figure> In restriction, the enzyme cleaves the DNA 25 to 26 base-pairs 3′ to this sequence to leave single-stranded 5′ protrusions two bases long.</td>
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Keywords:To whom correspondence should be addressed  
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