PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae |
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Authors: | A T Carter F Beiche B Hove-Jensen A Narbad P J Barker L M Schweizer and M Schweizer |
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Institution: | (1) Genetics and Microbiology Department, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK, GB;(2) Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, 83H S?lvgade, DK-1307 Copenhagen K, Denmark, DK;(3) The Babraham Institute, Microchemical Facility, Cambridge CB2 4AT, UK, GB |
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Abstract: | In Saccharomyces cerevisiae the metabolite phosphoribosyl-pyrophosphate (PRPP) is required for purine, pyrimidine, tryptophan and histidine biosynthesis.
Enzymes that can synthesize PRPP can be encoded by at least four genes. We have studied 5-phospho-ribosyl-1(α)-pyrophosphate
synthetases (PRS) genetically and biochemically. Each of the four genes, all of which are transcribed, has been disrupted
in haploid yeast strains of each mating type and although all disruptants are able to grow on complete medium, differences
in growth rate and enzyme activity suggest that disruption of PRS1 or PRS3 has a significant effect on cell metabolism, whereas disruption of PRS2 or PRS4 has little measurable effect. Using Western blot analysis with antisera raised against peptides derived from the non-homology
region (NHR) and the N-terminal half of the PRS1 gene product it has been shown that the NHR is not removed by protein splicing. However, the fact that disruption of this
gene causes the most dramatic decrease in cell growth rate and enzyme activity suggests that Prs1p may have a key structural or regulatory role in the production of PRPP in the cell.
Received: 15 July 1996 / Accepted: 24 October 1996 |
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Keywords: | Phosphoribosylpyrophosphate synthetase Gene family Nucleotide metabolism Saccharomyces cerevisiae |
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