Biosynthesis of brassinolide from teasterone via typhasterol and castasterone in cultured cells of Catharanthus roseus |
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| Authors: | Hiroyuki Suzuki Shozo Fujioka Suguru Takatsuto Takao Yokota Noboru Murofushi Akira Sakurai |
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| Institution: | (1) The Institute of Physical and Chemical Research (RIKEN), Wako-shi, 351-01 Saitama, Japan;(2) Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan;(3) Department of Chemistry, Joetsu University of Education, Joetsu-shi, 943 Niigata, Japan;(4) Department of Biosciences, Teikyo University, 320 Utsunomiya, Japan |
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| Abstract: | The biosynthesis of brassinolide (BL) in crown gall and nontransformed cells of Catharanthus roseus in which BL, castasterone (CS), typhasterol (TY), and teasterone (TE) are endogenous was investigated using deuterated TY and TE as substrates. The metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS) and/or GC-selected ion monitoring (SIM). It was found that these cells converted TY to CS and BL, as well as TE to TY and CS. Because the pathway from CS to BL in the cells has already been confirmed, a biosynthetic sequence of TE  TY CS BL was established. Reversible conversion between TE and TY was observed.Biosynthesis of brassinosteroids in Catharanthus roseus. Part III. Part II of this series: Suzuki et al. (1993). |
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