The effect of the suspensor and gibberellic acid on Phaseolus vulgaris embryo protein synthesis |
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Authors: | E D Walthall T Brady |
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Affiliation: | 1. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph ON, Canada, N1G 5C9;2. Department of Human Health and Nutritional Sciences, University of Guelph, Guelph ON, Canada, N1G 2W1;3. Department of Plant Agriculture, University of Guelph, Guelph ON, Canada, N1G 2W1;4. Department of Pathobiology, University of Guelph, Guelph ON, Canada, N1G 2W1 |
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Abstract: | The role of the suspensor in the early development of the dicot embryo has been described as merely an anchor or, conversely, as the major route of nutrients into the embryo. In order to further elucidate the role of the suspensor we have examined protein synthesis in early 0.2-mm and late heart stage 0.5-mm Phaseolus vulgaris (var. Taylor's Horticultural) embryos in tissue culture. Protein synthesis was examined in embryos and suspensors. Our results showed that in 0.2-mm embryos virtually all protein synthesis was dependent on an attached suspensor. Maximum protein synthesis in 0.5-mm embryos was observed when embryos were cultured attached to the suspensor. The levels were moderately decreased when the embryo was cultured detached from or without the suspensor. Gibberellic acid at 10(-6) to 10(-7) M elicited the same protein diversity and greater [35S]methionine incorporation than did the attached suspensor in 0.2-mm embryos. Embryos of 0.5 mm did not appear to be differentially responsive to various gibberellin concentrations. |
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