Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10 |
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Authors: | R Morona P Reeves |
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Institution: | (1) Department of Microbiology and Immunology, The University of Adelaide, 5000 Adelaide, South Australia |
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Abstract: | Summary We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.Abbreviations Tris
Tris (hydroxymethyl) aminomethane
- EDTA
Ethylenediamine tetra-acetic acid
- DOC
Sodium deoxycholate
- DNA
Deoxyribonucleic acid
- SDS
Sodium dodecyl sulphate
- kb
kilo base pairs |
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Keywords: | |
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