Establishment of EGF, EGF-R and FN-R positive human adenocarcinoma cell line (GAC-1)] |
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| Authors: | Y Ohmachi A Murata N Matsuura T Yasuda T Yasuda N Takata Y Oka N Hiraoka J Nishijima H Oka |
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| Institution: | Department of Surgery, Osaka University Medical School, Japan. |
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| Abstract: | A human adenocarcinoma cell line designated as GAC-1, was established from ascites of the 56-year old male patient with rapidly progressive gastric cancer. The doubling time was about 18.5 hours in vitro, and cell cycle analysis using flow cytometry showed marked increase of S phase (46.1%). Immunohistochemical demonstration of GAC-1 cells revealed positive staining of TGF-alpha, EGF, EGF-R, FN-R, laminin and negative staining of fibronectin. Histogram of them indicated aneuploidy with modal number 57 and they formed tumors in nude mice. |
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