Radiochemical high-performance liquid chromatography detection of arginine metabolism in human endothelial cells |
| |
Authors: | Barilli Amelia Visigalli Rossana Rotoli Bianca Maria Bussolati Ovidio Gazzola Gian Carlo Parolari Alessandro Dall'Asta Valeria |
| |
Institution: | Dipartimento di Medicina Sperimentale, Sezione di Patologia Generale e Clinica, Università degli Studi di Parma, 43125 Parma, Italy. |
| |
Abstract: | Arginine is a semi-essential amino acid that plays an important role in the regulation of metabolic processes associated with several pathological/physiological conditions. In the vasculature, it mainly exerts its biological functions as a substrate of two alternative pathways: the conversion to nitric oxide (NO) by nitric oxide synthase (NOS) and the breakdown to urea and ornithine by arginase. To determine arginine metabolism, in the current study we propose an original radiochemical technique that allows the simultaneous monitoring of NOS and arginase activation within intact cells. Taking advantage of this method, we show here the consequences of different experimental conditions known to modulate endothelial homeostasis on arginine metabolism. |
| |
Keywords: | Glycated hemoglobin Amperometric biosensor Fructosyl amino acid oxidase Zinc oxide nanoparticles Polypyrrole |
本文献已被 ScienceDirect PubMed 等数据库收录! |