Responses of mammalian metaphase chromosomes to endonuclease digestion |
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| Authors: | Sahasrabuddhe C G Pathak S Hsu T C |
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| Institution: | (1) Department of Biochemistry, The University of Texas System Cancer Center M.D. Anderson Hospital and Tumor Institute, 77030 Houston, Texas, USA;(2) Department of Biology, The University of Texas System Cancer Center M.D. Anderson Hospital and Tumor Institute, 77030 Houston, Texas, USA |
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| Abstract: | Digestion of fixed metaphase chromosomes by endonucleases (micrococcal nuclease and DNase II) under optimal digestion conditions followed by Giemsa staining produces sharp banding patterns identical to G-bands. In 3H-thymidine labeled, synchronized metaphase cells of the chinese hamster (CHO line), the band induction is accompanied by the removal of DNA. The single strand specific nuclease S1 and DNase I do not produce such banding patterns. |
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