A combined pathway of sulfur compound disproportionation in Desulfovibrio desulfuricans |
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Authors: | Heribert Cypionka ,rea M. Smock,Michael E. Bö ttcher |
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Affiliation: | Departamento de Microbiologı́a y Parasitologı́a, Facultad de Biologı́a, Universidad de Santiago de Compostela, Santiago de Compostela 15706, Spain;Departamento de Microbiologı́a, Facultad de Biologı́a, Universidad de Valencia, Burjassot 46100, Spain |
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Abstract: | The fates of the two different sulfur atoms of the thiosulfate molecule during anaerobic disproportionation by the sulfate-reducing bacterium Desulfovibrio desulfuricans were followed by isotope mass spectrometry. During disproportionation, 32S-thiosulfate was preferentially metabolized, and the residual thiosulfate became enriched in 34S. The sulfate formed was isotopically heavier than the inner sulfur of the consumed thiosulfate. Vice versa, the sulfide formed was isotopically lighter than the outer sulfur of the consumed thiosulfate. These results indicate that thiosulfate is cleaved to intermediates that undergo further disproportionation to sulfate and sulfide in a second step. These intermediates are probably elemental sulfur and sulfite. It is concluded that disproportionation of thiosulfate, sulfite and elemental sulfur includes a combined pathway. |
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Keywords: | Photobacterium damselae subsp. damselae Vibrio damsela Starvation Non-culturable state Cellular change |
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