Sequence and chromosomal localisation of the gene encoding ovine latent transforming growth factor-β1 |
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| Authors: | Christopher J Woodall Lorna J McLaren Neil J Watt |
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| Institution: | Department of Veterinary Pathology, Summerhall, University of Edinburgh, Edinburgh EH9 1QH, UK |
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| Abstract: | Transforming growth factor-β1 (TGF-β1) plays important roles in pathologic processes. To further investigate the actions of this cytokine in sheep, the entire 1170-bp ovine TGF-βl pro-protein-encoding sequence has been determined by the cloning and sequencing of specific polymerase-chain-reaction amplification products of TGF-β1 cDN A sequences. In addition, these sequences have been used to estimate the length of the TGF-β1 mRNA as 1.5-1.7 kb by Northern blot hybridization and determine that the ovine TGF-β1 gene occupies a single locus in the sheep genome by chromosomal in situ hybridization. |
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| Keywords: | Recombinant DNA cytokine pathology PCR homology to porcine murine and human genes |
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