中国野生葡萄组织培养研究

对中国野生山葡萄左山—1、左山—2、燕山葡萄燕山—1和秋葡萄平利—7的叶片、叶柄、茎段及单芽茎段进行了离体培养研究。诱导左山—1叶片分化出不定芽的培养基为MS BA 5．0mg／L NAA0．1mg／L，诱导率2．5％；诱导平利—7叶柄分化出不定芽的培养基为MS BA7．0mg／L NAA0．1mg／L，诱导率1．95％；诱导左山—1、燕山—1和平利—7茎段分化出不定芽的培养基与叶柄相同，但诱导率相对较高，分别为8．25％、4．88％和6．49％；应用这一培养基对平利—7、左山—2的单芽茎段进行培养，丛状不定芽的诱导率均为100％。不定芽继代培养基为MS BA0．5mg／L IBA0．2mg／L；生根培养基为1／2MS IBA0．1—0.2mg／L。

Tissue culture of chinese wild grape

The leaf ,petiole, stem-segment and stem-segment with single bud of the chinese wild grapes,Zuoshan-1, Zuoshan-2 (V. amurensis),Yanshan-1 (V. yeshanensis) and Pingli-7 (V. romanetii) were in vitro cultured. The optimal medium for the leaf of Zuoshan-1 was MS+BA 5. 0 mg/L+NAA 0. 1 mg/L,and the inducing rate of adventitious buds was 2. 5%. The most effective medium for inducing adventitious buds from the petioles of Pingli-7 was MS+BA 7. 0 mg/L+NAA 0. l mg/L,and the inducing rate was 1. 95%. The optimum medium for the stem-segments inducing of Zuoshan-1,Yanshan-1 and Pinli-7 was MS+BA 7. 0 mg/L+NAA 0. 1 mg/L and the rate was 8. 25% ,4. 88% and 6. 49% respectively. Furthermore,over-grown adventitious buds were induced from stem-segments with single bud of Pingli-7,Zuoshan-2 in this medium and the percentage was all 100%. The subculture medium was MS+BA 0. 5 mg/L+IBA 0. 2 mg/ L. The rooting medium was 1/2 MS+IBA 0. 1-0. 2 mg/L.