Evaluation of postharvest-processed oysters by using PCR-based most-probable-number enumeration of Vibrio vulnificus bacteria |
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| Authors: | Wright Anita C Garrido Victor Debuex Georgia Farrell-Evans Melissa Mudbidri Archana A Otwell W Steven |
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| Affiliation: | University of Florida, Department of Food Science and Human Nutrition, P.O. Box 110370, Gainesville, FL 32611, USA. acw@ufl.edu |
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| Abstract: | Postharvest processing (PHP) is used to reduce levels of Vibrio vulnificus in oysters, but process validation is labor-intensive and expensive. Therefore, quantitative PCR was evaluated as a rapid confirmation method for most-probable-number enumeration (QPCR-MPN) of V. vulnificus bacteria in PHP oysters. QPCR-MPN showed excellent correlation (R(2) = 0.97) with standard MPN and increased assay sensitivity and efficiency. |
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