Bar to normal UGA translation by the selenocysteine tRNA. |
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| Authors: | W Q Li M Yarus |
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| Affiliation: | Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347. |
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| Abstract: | The selC gene product, tRNA(Sec), inserts selenocysteine at UGA (opal) codons in a specialized mRNA context. We have investigated the action of the tRNA at ordinary UGA codons, normally not translated, by changing the unusual structural features of tRNA(Sec). Sequences in the D arm, CCA arm and variable arm of the tRNA all contribute to the prohibition against translation of ordinary UGA codons. One multiple mutant is a moderately efficient serine-inserting UGA suppressor tRNA. |
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