Yeast silencers create domains of nuclease-resistant chromatin in an SIR4-dependent manner |
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Authors: | Susan K Reimer Andrew R Buchman |
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Institution: | (1) Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA, US |
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Abstract: | Previous analysis of the repression of the silent mating type loci in Saccharomyces cerevisiae has linked the mechanism of silencing to the formation of a chromatin domain at the silenced loci. In this study, a TRP1 reporter gene was used to examine changes in chromatin structure in a neutral environment. This enabled the chromatin structure
organized by yeast silencers to be compared directly with changes effected by the yeast α2 repressor. It was found that silencers
mediate the formation of lengthy nuclease-resistant domains on the DNA, rather than specifically positioning nucleosomes over
promoter regions as the α2 repressor does. Silencing at the TRP1 reporter gene closely resembled silencing at the HMR and HML loci. Repression of the test gene was optimal when two silencers flanking the reporter gene were used, mimicking the situation
at the silent loci. In addition, both repression of the reporter gene and the formation of nuclease-resistant chromatin domains
was SIR4 dependent.
Received: 31 October 1996; in revised form: 6 March 1997 / Accepted: 25 March 1997 |
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