Double-membraned Liposomes Sculpted by Poliovirus 3AB Protein |
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Authors: | Jing Wang Jennifer B. Ptacek Karla Kirkegaard Esther Bullitt |
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Affiliation: | From the ‡Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118 and ;the §Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94301 |
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Abstract: | Infection with many positive-strand RNA viruses dramatically remodels cellular membranes, resulting in the accumulation of double-membraned vesicles that resemble cellular autophagosomes. In this study, a single protein encoded by poliovirus, 3AB, is shown to be sufficient to induce the formation of double-membraned liposomes via the invagination of single-membraned liposomes. Poliovirus 3AB is a 109-amino acid protein with a natively unstructured N-terminal domain. HeLa cells transduced with 3AB protein displayed intracellular membrane disruption; specifically, the formation of cytoplasmic invaginations. The ability of a single viral protein to produce structures of similar topology to cellular autophagosomes should facilitate the understanding of both cellular and viral mechanisms for membrane remodeling. |
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Keywords: | Lipoprotein Liposomes Membrane Bilayer Membrane Biophysics Membrane Reconstitution Positive-strand RNA Viruses Viral Protein Viral Replication |
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