Poly(methylene co-guanidine) coated alginate as an encapsulation matrix for urease |
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Authors: | Elizabeth Hearn Ronald J Neufeld |
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Institution: | Department of Chemical Engineering, Queen's University, Kingston, Ont., Canada K7L 3N6 |
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Abstract: | Urease was encapsulated within alginate beads, coated with poly(methylene co-guanidine) membranes via polyelectrolyte complexation. Membrane thickness increased with reaction time to 53 μm after 80 min, and to 59 μm with an increase in co-guanidine concentration from 2.5 to 20 mg ml−1. A 70% mass and 31% activity yield of urease resulted following encapsulation. Although co-guanidine strongly inhibited freely soluble urease (I0.5=5.8 μg ml−1 co-guanidine), immobilization stabilized the enzyme against inactivation. Encapsulated activity declined as the polycation concentration used for membrane formation increased; however an activity loss of only 35% was observed when the co-guanidine concentration was as high as 5 mg ml−1. Glucose protected against inactivation, with 0.5 increasing to 28.5 μg ml−1 for the freely soluble enzyme. When the beads were coated with co-guanidine in the presence of glucose, encapsulated urease activity was fully retained. |
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Keywords: | Alginate Poly(methylene co-guanidine) Urease Encapsulation |
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