Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli |
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Authors: | Wong H H Kim Y C Lee S Y Chang H N |
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Institution: | Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea. |
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Abstract: | The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coli was investigated. Cells were cultured in an exponential fed-batch mode to the OD600 of ca. 100 (48 gDCW/L) prior to induction. Six different post-induction nutrient feeding strategies (pH-stat, exponential, constant and linear change in feeding rate with three different slopes) were then applied, and bioadhesive protein production was examined. It was found that post-induction cell growth was independent of nutrient feeding rate. However, bioadhesive protein production was significantly affected by post-induction feeding strategies. Linearly changing post-induction feeding rate with a suitable slope allowed production of bioadhesive protein up to 5.3 g/L, which was higher than that obtained by the other post-induction feeding strategies. |
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