Molecular cloning and expression of the C-terminus of spider flagelliform silk protein from <Emphasis Type="Italic">Araneus ventricosus</Emphasis> |
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Authors: | Kwang Sik Lee Bo Yeon Kim Yeon Ho Je Soo Dong Woo Hung Dae Sohn Byung Rae Jin |
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Institution: | (1) College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea;(2) School of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, Korea;(3) Department of Plant Medicine, Chungbuk National University, Cheongju, 361-763, Korea |
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Abstract: | A cDNA coding for the C-terminus of spider flagelliform silk protein (AvFlag) was cloned from Araneus ventricosus. Analysis of the cDNA sequence shows that the C-terminus of AvFlag consists of 167 amino acids of a repetitive region and
87 amino acids of a C-terminal non-repetitive region. The peptide motifs found in spider flagelliform silk proteins, GPGGX
and GGX, were conserved in the repetitive region of AvFlag. Phylogenetic analysis further confirmed that AvFlag belongs to
the spider flagelliform silk proteins. The AvFlag cDNA was expressed as a 28 kDa polypeptide in baculovirus-infected insect
cells. As a new expression approach for spider silk protein, the combination of polyhedrin and AvFlag creates a polyhedrin
AvFlag fusion protein (61 kDa) that is produced as recombinant polyhedra; this provides a basis for the source of spider silk
proteins for various applications. |
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Keywords: | Araneus ventricosus baculovirus expression vector cDNA cloning flagelliform silk protein spider |
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