Lactococcus lactis SpOx spontaneous mutants: a family of oxidative-stress-resistant dairy strains |
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Authors: | Rochat Tatiana Gratadoux Jean-Jacques Corthier Gérard Coqueran Bérard Nader-Macias Maria-Elena Gruss Alexandra Langella Philippe |
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Affiliation: | Unité de Recherches Laitières et de Génetique, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France. |
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Abstract: | Numerous industrial bacteria generate hydrogen peroxide (H(2)O(2)), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H(2)O(2). Three SpOx mutants displayed greater H(2)O(2) resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H(2)O(2)-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected. |
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