Adherence of Lactobacillus to Intestinal 407 Cells in Culture Correlates with Fibronectin Binding |
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Authors: | Darrell R Kapczynski Richard J Meinersmann Margie D Lee |
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Institution: | (1) Southeast Poultry Research, USDA/ARS, Athens, GA, USA, US;(2) Poultry Processing and Meat Quality Research, USDA/ARS, Athens, GA, USA, US;(3) Department of Medical Microbiology and Parasitology, The University of Georgia, Athens, GA 30602, USA, US |
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Abstract: | Lactobacilli are members of the normal mucosal microflora of most animals. Many isolates of Lactobacillus spp. are adherent to epithelial cells. In this study, using Lactobacillus acidophilus and L. agilis, we detected adherence in a pattern that suggested that the bacteria were binding to extracellular matrix proteins. Fluorescent
microscopy, by using anti-fibronectin antibody, demonstrated that the isolates localize in those areas where fibronectin was
detected. In addition, fibronectin pretreatment of the bacterial cells decreased adherence to Intestinal 407 epithelial cell
monolayers. Cellular binding to fibronectin was detected by enzyme-linked immunosorbent assay and affinity binding to radio-labeled
fibronectin. Fibronectin may be one of the eukaryotic receptors mediating attachment of Lactobacillus to mucosal surfaces.
Received: 19 January 2000 / Accepted: 2 March 2000 |
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