Tryptophan and indole-3-acetic acid accumulation in Acer cell cultures and its relationship with cell autolysis |
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| Authors: | Maurice M Moloney Malcolm C Elliott |
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| Institution: | (1) School of Life Sciences, Leicester Polytechnic, P.O. Box 143, LE1 9BM Leicester, UK;(2) Present address: Institute of Plant Biology of the University, 6, place Riponne, CH-1005 Lausanne, Switzerland |
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| Abstract: | The accumulation and decline of  free indole-3-acetic acid (IAA) and tryptophan has been monitored in cells of Acer pseudoplatanus L. grown in batch suspension cultures. The period of maximal IAA accumulation per cell or per unit dry weight of tissue was found to precede the peak of tryptophan accumulation by several days. A study of cell viability throughout a growth passage indicated the presence of a basal level of non-viable cells of 5–7%, with only minor increases occurring during the first week of the three-week growth passage. The results suggest that IAA biosynthesis is not regulated by substrate availability arising from proteolysis in dead cells.Abbreviation GC-MS
Gas chromatography-mass spectrometry
- IAA
indole-3-acetic acid
- 5-MT
5-methyltryptophan
- TLC
thin-layer chromatography |
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| Keywords: | Acer Auxin biosynthesis Cell culture Cell death (in culture) Tryptophan |
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