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Monitoring the reproductive status of Japanese monkeys (Macaca fuscata) by measurement of the steroid hormones in fecal samples |
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| Authors: | Takashi Yoshida Mie Matsumuro Sachiko Miyamoto Yasuyuki Muroyama Yasuko Tashiro Yuji Takenoshita Tadashi Sankai |
| Institution: | (1) National Institute of Infectious Diseases, Tsukuba Primate Center, 1 Hachimandai, Tsukuba, 305-0843 Ibaraki, Japan;(2) Biological and Environmental Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Saitama, 338-8570 Saitama, Japan;(3) National Institute of Infectious Diseases, Tsukuba Primate Center, 1 Hachimandai, Tsukuba, 305-0843 Ibaraki, Japan;(4) Primate Research Institute, Kyoto University, Kanrin, Inuyama, 484-8506 Aichi, Japan;(5) Laboratory of Human Evolution Studies, Department of Zoology, Kyoto University, 606-8502 Kyoto, Japan;(6) National Institute of Infectious Diseases, Tsukuba Primate Center, 1 Hachimandai, Tsukuba, 305-0843 Ibaraki, Japan |
| Abstract: | The menstrual cycles as well as the pregnancy in female Japanese monkeys (Macaca fuscata) were monitored by measuring the fecal estradiol concentrations and relative amounts of fecal progesterone. Steroids from fecal samples were extracted by using a previously developed simplified two-step method and then measured by radioimmunoassay. We successfully demonstrated that the two-step method is effective and convenient for monitoring the reproductive status of Japanese monkeys. |
| Keywords: | Menstrual cycle Pregnancy Fecal steroid Japanese monkey |
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